Downstream Bioprocessing Calculations and Strategies

ISPE CEUs*: 1.2
Type: Classroom Training Course

Dates and Locations

Description

Biotechnology is the fastest growing segment of the Life Sciences Industry today. It is essential that bioprocesses are designed and operated to maximize the yield and purity of the bioproduct.

This course will provide basic principles related to the downstream processing of the bioproduct. The goal of the course is to promote understanding of the development and use of working equations and theory pertaining to the isolation and purification of bioproducts produced in fermentors / bioreactors. This course will discuss the different unit operations that are implemented in downstream processing to isolate and purify products.

We recommend that participants without a background in biologocal sciences complete the Upstream Bioprocessing Calculations course prior to attending Downstream Bioprocessing Calculations and Strategies.

The course will present information on:

• Types of centrifuges and filters
• Particle settling during centrifugation
• Flux reduction due to cake formation during dead-end filtration
• Fouling and concentration polarization during crossflow filtration
• Particle growth during precipitation and crystalization
• Phase partioning during extraction
• Isotherms describing solute adsorption
• Batch versus continuous extraction and adsorption processes
• Types of chromatography (IEX, HIC etc..)
• Breakthrough curves during fixed-bed adsorption
• Peak spreading and peak separation during elution chromatography
• Non-linear isotherm effects of peak shapes during elution chromatography

The course also will address the differences between the unit operations used to isolate and to purify the bioproduct. Participants will receive illustrations of biological and process systems, and a summary of terms and working equations.

In addition, participants will be led through concise derivations of the working equations and example problems that illustrate use of the equations. Specific discussions on minimizing the number of process steps by and choosing the optimum sequence of steps will be explored for different types of biomolecules from antibiotics to antibodies.

Take Back Your Job

• Recognize different types of centrifuges and filtration equipment
• Estimate comparable settling conditions across different centrifuge types
• Estimate permeate flux rates for cross-flow and dead end filtration
• Estimate time requirements for batch precipitation and crystallization
• Estimate crystal size and yields for continuous crystalizers
• Estimate yield/purity of bioproducts following single and multiple stage extractions
• Estimate yield/purity of bioproducts following batch and continuous adsorption steps
• Distinguish between different chromatography modes (IEX, HIC etc..)
• Predict breakthrough times during fixed bed adsorption
• Predict the elution time, peak shape, resolution and yield of chromatography processes

Attendance Suggested For

This course is intended to be taken by individuals within the sciences or engineering fields and who have an interest in using theoretical and practical equations to model and/or improve specific unit operations (i.e. centrifugation, filtration, extraction, crystallization and chromatography etc.) and/or devise optimal unit operation sequences.