ISPE Glossary of Pharmaceutical and Biotechnology Terminology

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ELISPOT (Enzyme-Linked Immunosorbent Spot assay)

A membrane-bottom plate ELISA assay where an antibody against a specific cytokine is bound to a membrane instead of a styrene plate. Then cells are added and if they produce the appropriate cytokine, it will bind the antibodies on the membrane. After washing away the cells, a second antibody against the cytokine is added that is labeled and will show a spot where the secreting cells lie. Often the membrane is punched out and analyzed under a microscope.

See also: Cytokine Assay

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