ISPE Glossary of Pharmaceutical and Biotechnology Terminology

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The migration of electrically charged proteins, colloids, molecules, or other particles when dissolved or suspended in an electrolyte through which an electric current is passed. The most important use of electrophoresis is in the analysis of blood proteins. Since the proportion of these proteins varies widely in different diseases, electrophoresis can be used for diagnostic purposes. Electrophoresis is used to study bacteria and viruses, nucleic acids, and some types of smaller molecules, including amino acids.

See also: Agarose Gel Electrophoresis Gel Electrophoresis Immuno Electrophoresis


A technique that separates charged molecules – such as DNA, RNA or protein – on the basis of relative migration in an appropriate matrix (such as agarose gel or polyacrylamide gel) subjected to an electric field.

Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999

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