ISPE Glossary of Pharmaceutical and Biotechnology Terminology

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Four-Base Cutter; Four-Base-Pair-Cutter; Four-Cutter

A type II restriction endonuclease that binds (and subsequently cleaves) DNA at sites that contain a sequence of four nucleotide pairs that is uniquely recognized by that enzyme. Because any sequence of four bases occurs more frequently by chance than any sequence of six bases, four-base cutters cleave more frequently than do six-base cutters. Thus, four-base cutters create smaller fragments than six-base cutters.

Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999

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