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Any prokaryotic organism that does not retain the first stain (crystal violet) used in Gram’s staining technique. It does retain the second stain (safranin O) and therefore has a pink color when viewed under a light microscope. Retention of the stain is due to the structure of the cell wall.
Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999
See also: Gram’s Stain
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