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Sometimes called high-performance liquid chromatography, is a separation technique based on a solid stationary phase and a liquid mobile phase. Separations (into distinct bands) are achieved by partition, adsorption, or ion-exchange processes, depending upon the type of stationary phase used. Each band is then profiled as the solvent flows through a UV detector, or by fluorescence, or refractive index detectors.
Liquid chromatography performed at high pressures (as high as 8,000 psi) with small-particle packings (as small as 3µm in diameter). HPLC methods include reverse-phase, normal-phase, size-exclusion, ion-exchange, ion, chiral, hydrophobic-interaction, and hydrophilic-interaction chromatography. HPLC has been used since the late 1960s, whereas liquid chromatography has been in use since 1903.
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