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The temperature at which a doublestranded DNA or RNA molecule denatures into separate single strands. The Tm is characteristic of each DNA species and gives an indication of its base composition. DNAs rich in G:C base pairs are more resistant to thermal denaturation than A:T rich DNA since three hydrogen bonds are formed between G and C, but only two between A and T.
Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999
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