ISPE Glossary of Pharmaceutical and Biotechnology Terminology

Print this page         
Find Definition by Term and/or Language
Term Language (Optional)
Browse All Terms
Beginning With By Language
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z :: All English
Deutsch
Francais

Trypsin, Tryptic Digestion

Trypsin allows the growth of cells as independent microorganisms distinct from tissue culture by causing cell disaggregation. Excised tissue is softened and treated with a proteolytic enzyme, normally trypsin, then washed and suspended in a growth medium to produce a primary culture. Subculturing from the primary culture usually involves treatment with antitrypsin (such as serum) to produce a secondary culture. Cell lines are established by repeated culture through cycles of growth, trypsinization, and subculture. Trypsin is also used to remove anchorage-dependent cells from their attached substratum.

Do you have a term that should be included in the glossary?
Submit a term for review

My ISPE

  • Click to go to My Communities of Practice
  • Click to go to My Affiliate or Chapter
  • Click to go to My Profile
Click to go to the Member Gift