ISPE Glossary of Pharmaceutical and Biotechnology Terminology

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cDNA (Complementary DNA)

The double-stranded DNA complement of an mRNA sequence; synthesized in vitro from a mature RNA template using reverse transcriptase (to create a single strand of DNA from the RNA template) and DNA polymerase (to create the double-stranded DNA). Preparation of cDNAs is often the first step in cloning DNA sequences of interest. Used as specific and sensitive probes in hybridization studies, because cDNAs usually do not include regulatory or other controlling sequences, and so they can be used to identify (probe) and isolate genes and their associated sequences from genomic DNA.

Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999

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