ISPE Glossary of Pharmaceutical and Biotechnology Terminology

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Gene Cloning

The process of synthesizing multiple copies of a particular DNA sequence using a bacteria cell or another organism as a host. The gene of interest is inserted into a self-replicating DNA molecule (DNA vector, often a plasmid) and the resulting recombinant DNA molecule is amplified in an appropriate host cell. Used in genetic engineering. Also known as Molecular Cloning; Cloning.

Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999

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