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An enzyme that specifically degrades RNA or singlestranded DNA to 5’ mononucleotides. Purified from the filamentous fungus Aspergillus oryzae, S1 nuclease is used in assessing the extent of a hybridization reaction by removing unpaired regions. It is also used to remove the sticky ends of restriction fragments. In S1 mapping, the coding region of a gene is detected by performing mRNA-DNA hybridization and removing unpaired DNA with S1 nuclease.
Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999
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